Molecular detection of Vibrio cholerae and Vibrio parahaemolyticus from healthy broilers and backyard chickens for the first time in Bangladesh- A preliminary study

Many of the Vibrio spp. are major public health concerns globally. cholerae and parahaemolyticus etiology pandemic epidemic diarrhea foodborne illness, respectively. Poultry has potential to harbor pathogenic spp., which can have a detrimental impact on if they transmitted humans. We, therefore, screened 54 cloacal swab samples from healthy chickens (broiler=27, backyard= 27) detect V. parahaemolyticus. were isolated identified by culturing, biochemical tests, PCR, antibiogram profiles determined disk diffusion method. By 29.63% (16/54; 95% CI: 19.14-42.83%) positive for where backyard had significantly higher (p< 0.05) occurrence (44.44%; 27.59-62.69%) than broilers (14.82%; 5.92-32.48%). was found in 22.22% (6/27; 10.61-40.76%) chicken samples, dominant (0/27, 0%, 0.00-12.46%). In addition, 7.41% (2/27; 1.32-23.37%) broiler, 14.82% (4/27; 5.92-32.48%) samples. The toxR gene all isolates, suggesting presence other virulence genes, whereas no isolates contained tdh gene. Isolated high moderate resistance tetracycline, azithromycin, erythromycin, streptomycin. antibiotic-resistant broiler is concern because possibility food chain contamination